Considerations To Know About cytometers
Considerations To Know About cytometers
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Information-enriched characterization of mobile populations. Morphological information and facts from pictures can add on the richness of flow cytometry facts. As an example, the figure demonstrates an in any other case standard apoptosis assay applying Annexin V and PI, introducing mobile imaging to characterize cells in Every single inhabitants to expose morphologically unique characteristics. These insights could not have already been attained from multiplex staining by itself.
As samples are obtained over the Attune CytPix Flow Cytometer, the higher-speed brightfield camera captures and stores pictures of detected gatherings in a rate nearly six,000 images for each second, dependant upon the flow amount and picture dimensions. For greater overall flexibility, Attune Cytometric Computer software allows you to change the image capture frequency as necessary and choose a particular gate for impression capture, if necessary.
(B) RAW 264.seven macrophages are sometimes annotated inaccurately with respect to particle count due to their complexity and noncircular borders. The quantity of singlets obtainable for Investigation improves after the consumer trains and employs a far more accurate model.
Find new insights into your experimental facts by visualizing populations applying merged fluorescence and morphology knowledge. Our computer software interface wizards enable downsampling, concatenation (merged information) and dimensionality tools which can be constructed to the application for an intuitive, streamlined data Investigation practical experience.
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In addition, it truly is illuminating to find out the amount of doublets slip through classic singlet gates, and terrific to confirm the position of unforeseen double-beneficial gatherings in my Examination."
Optimizing gating technique. Even sturdy manual singlet gating is mistake-inclined and stays a subjective decision stage in almost all flow cytometry assays. Imaging can be utilized to verify and adjust gates to include only solitary cells of interest.
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The Attune NxT cytometer is definitely the trustworthy workhorse instrument your lab can trust in for steady and trusted functionality. The Attune CytPix product contains a substantial-speed brightfield camera and AI-driven graphic analysis application, with new upgrades which include device Finding out applications and an intuitive consumer interface, enabling enhanced information precision, accuracy, and more rapidly insights into sample biology.
Perhaps most of all, these activities comprise cells of clearly distinctive phenotypes which could lead on to incorrect conclusions with regards to double beneficial occasions (specifically in rare populations).
Sound waves allow exact mobile alignment, leading to Fantastic facts high-quality and ~10X more quickly sample flow rates.
We Formerly shown the power of imaging Auto-T/Ramos cell interactions. Allow’s examine just the inhabitants of best interest, the double optimistic functions, to learn more. We could now use prolonged morphological parameters (here, circularity and skewness of depth) to more analyze the features of these populations and refine gating on these gatherings, escalating info robustness.
Leverage the power of Superior technologies and innovation to accelerate your discoveries. Trust in the Attune Flow Cytometer spouse and children to provide the reliability, velocity, and accuracy that set your operate apart.
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At the guts of each Attune Flow Cytometer lies our groundbreaking acoustic focusing technological innovation, which makes use of audio waves to meticulously align cells throughout the flow mobile. read more This revolutionary tactic surpasses classic hydrodynamic methods, leading to quicker processing speeds, Extraordinary knowledge excellent, and greater sample throughput.
As the mobile line experienced Earlier been analyzed on the Attune CytPix Flow Cytometer, the researchers went back again to the images and have been capable of document the microbial infection at the least 5 times before.
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What seemed to be a homogeneous inhabitants is really extra various—an insight that should be regarded as when interpreting final results.